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Back in 2011 while in the lab of Katharina Ribbeck I started working on a project funded by the Johnson and Johnson Corporation. At the time J&J provided us with funds to simply explore some aspects of mucosal health. Our goal was to better understand how our mucus layer works to protect us and to elaborate new strategies for mucosal repair. In addition to this “no string attached” funding model offered by J&J (which is rare today) we had great support from J&J staff. Julie Hirsch and Anthony Geonnotti were particularly involved and provided experimental data and valuable feedback throughout the project.

This work resulted in interesting insights in the special role of glycosylation in the hydration and lubrication properties of mucins. This is important since decreased and altered glycosylation of mucins occur in several diseases and can, for instance, lead to dry eye or dry mouth symptoms. But even more exciting to me was our attempt to compensate for the lost of glycans on mucins molecules.

While working on understanding the effect of mucin deglycosylation we established a model for defective mucins, which had a decreased ability to hydrate and lubricate surfaces. We decided to replace the missing glycan structures by synthetic polyethylene glycol (PEG) polymers. We got the PEG to stick to the mucins by using glycan-binding proteins (lectin) as anchors. Obviously PEG molecules are far from recapitulating the complex glycan structures decorating the mucin molecules, but it was close enough! The attachment of PEG to the altered mucin increased hydration and lubrication of mucins on surfaces.

This work took a while to be fully completed, and we owe a great deal to Prof. Oliver Lieleg and his group for helping bring much of the tribology data into the paper. The paper was published in September of 2015 in Advanced Materials Interfaces and is available in Open Access.  A patent was also granted earlier this year.

The core message of this story is told in this picture that, I hope, is accessible to most.  You can find this infographic (large scale) and several others in the outreach section of the website.

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  Posts

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March 27th, 2017

Get funded in Sweden. A guide for starting researchers.

February 23rd, 2017

The Swedish Foundation for Strategic Research awards a 5-year grant to the group

December 27th, 2016

New infographic on mucin repair

December 1st, 2016

2nd workshop on protein materials – March 9-10, 2017

October 31st, 2016

Opportunities with the KTH – CSC Programme

September 22nd, 2016

New PhD position – Here’s what you should know

September 14th, 2016

Grant proposal – Methylcellulose as next generation microbial culture substrate

August 10th, 2016

A call for better mucins

July 24th, 2016

Join us at the ESOF 2016, the EuroScience Open Forum!

April 6th, 2016

We will be at the World Biomaterials Congress in Montreal – May 2016